Ruud van Nistelrooy has told Holland coach Marco van Basten he is no longer available for the national team.
The 30-year-old Real Madrid striker, who has been capped 54 times by his country, has not featured at international level since last summer`s World Cup.
His announcement will come as a blow to van Basten, who thought he had built some bridges with the former Manchester United man.
'(It is) disappointing, I always played for Oranje with much pleasure and I would have liked to continue to do that,' said van Nistelrooy in a statement.
Van Basten revealed he and assistant John van 't Schip had held talks with the striker.
He said: 'We have had a good conversation on Friday in Madrid about the cooperation over the past year.
'We ironed out some folds. And with that there was a base to eventually come together again.'
Van Basten told van Nistelrooy he could not guarantee him a starting place in the side.
'We have told Ruud when he asked us about this, that the situation about his position is no longer the same as a year ago.
'In 2006, we saw other candidates for the striking position.
'That is why he should be prepared to compete with the others. We cannot guarantee him a starting place. That would be not be fair to the other candidates for the position.'
That standpoint was the obstacle for van Nistelrooy to make himself available for Holland again.
Van Nistelrooy said: 'I did not specifically ask for a place in the side, but I did want to have the feeling that I would be a candidate for that.
'I did not get that feeling. That is why I don't see the conditions to give a good performance. No-one benefits from frustration. That's why this was a difficult but correct decision.'